Polymerase chain reaction: A molecular diagnostic tool in periodontology

Polymerase Chain Reaction: a Powerful Diagnostic and Research Tool

To fully understand cellular processes, scientists often examine events at the level of nucleic acids and protein molecules. These studies are complicated by the fact that cells have miniscule amounts of molecules of interest which are too small to be seen. So we require molecular tools to visualize these molecules. Polymerase chain reaction is one such technique widely used in molecular biolog...

Polymerase chain reaction as a diagnostic tool for detecting Leishmania.

Polymerase chain reaction (PCR) has been used to identify a Leishmania parasite in a cutaneous ulcer from a 27-year-old patient infected during travel in Peru. The available classical diagnostic methods could not confirm the diagnosis in a sufficiently short time. Therefore, two sets of oligonucleotides were designed and with both of them fragments of the expected size were obtained. The sequen...

Real-time polymerase chain reaction: a novel molecular diagnostic tool for equine infectious diseases.

The focus of rapid diagnosis of infectious disease of horses in the last decade has shifted from the conventional laboratory techniques of antigen detection, microscopy, and culture to molecular diagnosis of infectious agents. Equine practitioners must be able to interpret the use, limitations, and results of molecular diagnostic techniques, as they are increasingly integrated into routine micr...

Polymerase Chain Reaction molecular diagnostic technology for monitoring chronic osteomyelitis

BACKGROUND Osteomyelitis is a devastating condition whose treatment relies on the detection of bacteria. The current standard of microbiology culture may not be adequate. Molecular biology based diagnostic procedures for detecting bacteria in orthopaedic infections was previously established, but has not been applied to the setting of chronic osteomyelitis. We aim to determine the applicability...

A Diagnostic Multiplex Polymerase Chain Reaction Assay for Ocular Herpetic Infections.